HTRA3 antibody (AA 112-144)

Catalog No. ABIN7870129

The Rabbit Polyclonal anti-HTRA3 antibody (ABIN7870129) specifically detects HTRA3 in WB and IHC (p). The antibody is reactive with Human and Rat samples.

Quick Overview for HTRA3 antibody (AA 112-144) (ABIN7870129)

Target: HTRA3 (HtrA Serine Peptidase 3 (HTRA3))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTRA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HTRA3 Antibody

Binding Specificity: AA 112-144

Purpose: HtrA3 Antibody

Purification: Purified

Immunogen: A portion of amino acids 112-144 from the human protein was used as the immunogen for the HtrA3 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the HtrA3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the HtrA3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for HTRA3

Target: HTRA3 (HtrA Serine Peptidase 3 (HTRA3))

Alternative Name: HtrA3

Background: Insulin-like growth factors (IGFs) stimulate the proliferation and differentiation of a vast number of cell types. The action of the growth factors is mediated and controlled by a complex system of components, including several proteases that cleave the IGF-Binding Proteins. HtrA1 is a 480 aa protein that contains an N-terminus homologous to MAC25 (IGFBP7) with a conserved Kazal-type serine protease inhibitor motif, as well as a C-terminal PDZ domain. Semiquantitative RT-PCR and immunoblot analyses showed an approximately 7-fold increase of PRSS11 in osteoarthritis cartilage compared with controls. HTRA2 is released from mitochondria and inhibits the function of XIAP by direct binding in a way similar to SMAC. Moreover, when overexpressed extramitochondrially, HTRA2 induced atypical cell death, which was neither accompanied by a significant increase in caspase activity nor inhibited by caspase inhibitors, including XIAP. A catalytically inactive mutant of HTRA2, however, did not induce cell death. Suzuki et al. (2001) concluded that HTRA2 is a SMAC-like inhibitor of IAP (inhibitor of apoptosis proteins) activity with a serine protease-dependent cell death-inducing activity.

UniProt: P83110