AHCY antibody (AA 112-432)

Catalog No. ABIN7870139

This Rabbit Polyclonal antibody specifically detects AHCY in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for AHCY antibody (AA 112-432) (ABIN7870139)

Target: AHCY (Adenosylhomocysteinase (AHCY))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AHCY antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-AHCY Antibody

Binding Specificity: AA 112-432

Purpose: AHCY Antibody / Adenosylhomocysteinase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human AHCY recombinant protein (Position: W112-Y432) was used as the immunogen for the AHCY antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the AHCY antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the AHCY antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for AHCY

Target: AHCY (Adenosylhomocysteinase (AHCY))

Alternative Name: AHCY

Background: AHCY antibody detects Adenosylhomocysteinase, an essential cytoplasmic enzyme encoded by the AHCY gene located on chromosome 20q11.22. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine (SAH) to adenosine and homocysteine, a key step in the methionine cycle that regulates cellular methylation potential. This enzyme is critical for maintaining the balance between methylation reactions and homocysteine metabolism in all mammalian cells. AHCY is ubiquitously expressed, with high levels in liver, kidney, brain, and heart where transmethylation reactions are most active.

Structurally, AHCY is a homotetrameric enzyme that binds NAD+ as a cofactor to mediate the reversible breakdown of SAH. It belongs to the adenosylhomocysteinase family of dehydrogenases, characterized by a conserved catalytic lysine and nicotinamide-binding motif. The enzyme undergoes conformational changes during catalysis, coupling the oxidation and hydrolysis of SAH to the regeneration of adenosine and homocysteine. AHCY operates at the intersection of methionine metabolism, DNA methylation, and nucleotide synthesis.

Functionally, AHCY plays a central role in controlling cellular methylation capacity by regulating the intracellular SAH concentration, a potent inhibitor of methyltransferases. By hydrolyzing SAH, AHCY maintains the S-adenosylmethionine (SAM)/SAH ratio required for normal methylation of DNA, RNA, proteins, and lipids. In liver, AHCY supports homocysteine recycling and methionine regeneration, while in neurons, it contributes to methylation-dependent neurotransmitter synthesis. The enzyme also interacts with metabolic partners such as MAT1A and CBS, forming a regulatory network that maintains one-carbon metabolism.

Mutations or deficiency in AHCY cause adenosylhomocysteinase deficiency, a rare metabolic disorder characterized by elevated SAH and global hypomethylation leading to developmental delay, muscle hypotonia, and hepatic dysfunction. Dysregulation of AHCY expression has also been linked to cardiovascular disease, liver fibrosis, and cancer. Pathway involvement includes methionine metabolism, homocysteine clearance, and methyl donor recycling. During development, AHCY expression ensures proper methylation patterns necessary for organogenesis and epigenetic regulation.

The AHCY antibody from NSJ Bioreagents is a valuable reagent for research into methylation biology, amino acid metabolism, and epigenetic regulation.

UniProt: P23526