Histone H2A antibody (AA 115-143)

Catalog No. ABIN7870200

The Mouse Monoclonal anti-Histone H2A antibody is suitable to detect Histone H2A in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for Histone H2A antibody (AA 115-143) (ABIN7870200)

Target: Histone H2A

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 938CT5-1-1

Product Details anti-Histone H2A Antibody

Binding Specificity: AA 115-143

Purpose: Histone H2AX Antibody / H2AFX

Purification: Protein G affinity

Immunogen: A portion of amino acids 115-143 from the human protein was used as the immunogen for the Histone H2AX antibody.

Isotype: IgG1

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Histone H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Histone H2AX antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Histone H2A

Target: Histone H2A

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

UniProt: P16104