ATG5 antibody (AA 118-155)

Catalog No. ABIN7870256

This Rabbit Polyclonal antibody specifically detects ATG5 in IHC (p). It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for ATG5 antibody (AA 118-155) (ABIN7870256)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATG5 Antibody

Binding Specificity: AA 118-155

Purpose: Zebrafish Atg5 Antibody / Autophagy protein 5

Purification: Antigen affinity chromatography

Immunogen: An E.coli-derived zebrafish Atg5 recombinant protein (amino acids S118-L155) was used as the immunogen for the Zebrafish Atg5 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Atg5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Atg5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: Atg5

Background: Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is also known as APG5 or ASP, and this gene is mapped to 6q21. It is found that knockdown of ATG5 in hepatocytes increased triglyceride levels with oleate or a second endogenous stimulus for triglyceride formation. These hepatocytes with ATG5 knockdown also had increased lipid droplet number and size. ATG5 is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.

UniProt: F1RDC0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy