Tricellulin antibody (AA 12-496)

Catalog No. ABIN7870297

The Rabbit Polyclonal anti-Tricellulin antibody (ABIN7870297) specifically detects Tricellulin in WB, ELISA, IHC and FACS. The antibody is reactive with Human and Rat samples.

Quick Overview for Tricellulin antibody (AA 12-496) (ABIN7870297)

Target: Tricellulin (MARVELD2)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tricellulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Tricellulin Antibody

Binding Specificity: AA 12-496

Purpose: MARVELD2 Antibody / Tricellulin

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MARVELD2 recombinant protein (Position: R12-H496) was used as the immunogen for the MARVELD2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MARVELD2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MARVELD2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Tricellulin

Target: Tricellulin (MARVELD2)

Alternative Name: MARVELD2

Background: MARVELD2 antibody detects MARVEL domain-containing protein 2, also known as Tricellulin, a tight junction-associated protein that maintains epithelial barrier integrity at tricellular junctions where three cells meet. MARVELD2 is essential for forming the barrier against paracellular diffusion of macromolecules, thereby preserving tissue polarity and homeostasis. The MARVELD2 antibody is widely used in epithelial biology and cell junction research to study tight junction architecture, permeability control, and barrier dysfunction.

MARVELD2 is encoded by the MARVELD2 gene located on human chromosome 5q13.1. The protein is approximately 558 amino acids long and contains four transmembrane segments with a cytoplasmic C-terminal tail. It localizes at tricellular tight junctions, distinct from bicellular tight junction proteins such as occludin and claudins. MARVELD2 interacts with angulin family proteins and zonula occludens proteins to anchor the tricellular junction complex to the actin cytoskeleton.

The MARVELD2 antibody detects a 65 kilodalton band by western blot and exhibits strong junctional staining along epithelial borders in immunofluorescence. MARVELD2 regulates diffusion barrier properties by sealing the tricellular junction core, ensuring that epithelial sheets restrict large solute movement. Loss of MARVELD2 disrupts paracellular sealing, resulting in barrier leakage and increased susceptibility to inflammation and infection.

Mutations in MARVELD2 cause autosomal recessive deafness (DFNB49) due to impaired tight junction formation in the cochlea, leading to hair cell degeneration and hearing loss. Altered expression is also associated with inflammatory bowel disease and cancer metastasis, where weakened epithelial integrity promotes tissue invasion and immune infiltration.

As a critical determinant of epithelial sealing and tissue organization, MARVELD2 serves as a biomarker for tight junction function and epithelial polarity. NSJ Bioreagents provides a validated MARVELD2 antibody optimized for its applications, supporting research into barrier formation, epithelial biology, and junctional signaling.

UniProt: Q8N4S9

Pathways: Sensory Perception of Sound, Cell-Cell Junction Organization