BCL10 antibody (AA 122-168)

Catalog No. ABIN7870331

This Mouse Monoclonal antibody specifically detects BCL10 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for BCL10 antibody (AA 122-168) (ABIN7870331)

Target: BCL10 (B-Cell CLL/lymphoma 10 (BCL10))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCL10 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM520

Product Details anti-BCL10 Antibody

Binding Specificity: AA 122-168

Purpose: Bcl10 Antibody

Purification: Protein G affinity chromatography

Immunogen: Amino acids 122-168 were used as the immunogen for this Bcl10 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The optimal dilution of the Bcl10 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Bcl10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for BCL10

Target: BCL10 (B-Cell CLL/lymphoma 10 (BCL10))

Alternative Name: Bcl10

Background: Bcl10, with an N-terminal caspase recruitment domain (CARD), is found in a number of apoptotic regulatory molecules. It was identified through its direct involvement in t(1,14) of mucosa-associated lymphoid tissue (MALT) lymphoma. Expression of Bcl10 was shown to induce NF-kB activation in a NIK-dependent pathway. This mAb labels subpopulations of normal B and T cells and is a useful tool for the sub-classification of lymphomas. In MALT lymphomas with the t(1,14) translocation, while 55 % of MALT lymphomas lacking this translocation exhibited the same labeling pattern, although at a much lower level.

UniProt: O95999

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins