SIN3B antibody (AA 127-1127)

Catalog No. ABIN7870399

The Rabbit Polyclonal anti-SIN3B antibody (ABIN7870399) specifically detects SIN3B in WB, ELISA, IF, IHC, ICC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SIN3B antibody (AA 127-1127) (ABIN7870399)

Target: SIN3B (SIN3 Transcription Regulator Homolog B (SIN3B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIN3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SIN3B Antibody

Binding Specificity: AA 127-1127

Purpose: SIN3B Antibody / Paired amphipathic helix protein Sin3b

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SIN3B recombinant protein (Position: Q127-E1127) was used as the immunogen for the SIN3B antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SIN3B antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SIN3B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SIN3B

Target: SIN3B (SIN3 Transcription Regulator Homolog B (SIN3B))

Alternative Name: SIN3B

Background: SIN3B antibody detects Paired amphipathic helix protein Sin3b, a transcriptional corepressor that regulates chromatin structure and gene expression by recruiting histone deacetylase (HDAC) complexes. SIN3B functions in concert with its paralog SIN3A to coordinate gene silencing, differentiation, and cell cycle control. The SIN3B antibody is widely used in epigenetics and transcriptional regulation research to examine HDAC-dependent gene repression.

SIN3B is encoded by the SIN3B gene on human chromosome 19p13.11. The protein is approximately 150 kilodaltons and contains multiple paired amphipathic helix (PAH) domains that mediate interactions with transcription factors and chromatin-modifying enzymes. SIN3B serves as a scaffold that assembles HDAC1, HDAC2, RBBP4/7, and other corepressors into multiprotein complexes targeting specific promoters.

The SIN3B antibody detects a 150 kilodalton band by western blot and shows nuclear localization by immunofluorescence. Through recruitment of HDAC complexes, SIN3B deacetylates histones H3 and H4, resulting in chromatin compaction and transcriptional repression. It also interacts with transcription factors such as E2F4, MAD, and REST, enabling context-dependent regulation of genes involved in proliferation, senescence, and neuronal development.

SIN3B plays key roles in differentiation, acting as a switch from proliferation to quiescence. Knockdown of SIN3B leads to uncontrolled growth and defective chromatin silencing, while overexpression suppresses oncogenic transcription programs. Altered SIN3B function has been implicated in cancer, diabetes, and neurodevelopmental disorders due to disrupted epigenetic control.

As a master regulator of chromatin remodeling, SIN3B integrates multiple signaling pathways to maintain transcriptional balance. NSJ Bioreagents provides a validated SIN3B antibody optimized for western blot, chromatin immunohistochemistry, and nuclear complex analysis, supporting research into epigenetic repression, differentiation, and tumor suppression.

UniProt: O75182