SESN3 antibody (AA 13-345)

Catalog No. ABIN7870470

The Rabbit Polyclonal anti-SESN3 antibody is suitable to detect SESN3 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF, ICC and FACS.

Quick Overview for SESN3 antibody (AA 13-345) (ABIN7870470)

Target: SESN3 (Sestrin 3 (SESN3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SESN3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SESN3 Antibody

Binding Specificity: AA 13-345

Purpose: SESN3 Antibody / Sestrin 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SESN3 recombinant protein (Position: N13-H345) was used as the immunogen for the SESN3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SESN3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SESN3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SESN3

Target: SESN3 (Sestrin 3 (SESN3))

Alternative Name: SESN3

Background: The SESN3 antibody targets Sestrin-3, a stress-responsive protein encoded by the SESN3 gene that acts as a key regulator of metabolism, oxidative stress, and autophagy. Sestrin-3 belongs to the sestrin family of highly conserved proteins that modulate AMPK and mTORC1 signaling in response to cellular stress and nutrient availability. By integrating metabolic and redox cues, Sestrin-3 protects cells from oxidative damage and supports energy balance. The SESN3 antibody provides a high-specificity reagent for studying these protective pathways and their dysregulation in metabolic and age-related diseases.

Sestrin-3 functions as both an antioxidant and a signaling adaptor. It promotes activation of AMP-activated protein kinase (AMPK) and inhibits mechanistic target of rapamycin complex 1 (mTORC1), thereby promoting autophagy and metabolic homeostasis under nutrient stress. The SESN3 antibody enables detection of this adaptive protein across a range of tissues, including liver, muscle, adipose, and brain, where it contributes to maintaining glucose and lipid metabolism. Through these pathways, Sestrin-3 serves as a critical mediator of cellular recovery following oxidative or genotoxic stress.

In metabolic research, Sestrin-3 has gained prominence as a regulator of insulin sensitivity and lipid catabolism. Elevated SESN3 expression improves glucose tolerance and suppresses lipogenesis through AMPK activation. Conversely, its loss results in impaired energy metabolism and increased susceptibility to metabolic syndrome. The SESN3 antibody supports studies of these mechanisms by quantifying protein levels in metabolic tissues and cell lines subjected to nutrient deprivation or stress stimuli.

Sestrin-3 also contributes to antioxidant defense by reducing reactive oxygen species (ROS) accumulation and supporting regeneration of peroxiredoxins. These functions are critical in preventing oxidative stressi 1/2induced damage in aging, cardiovascular disease, and neurodegeneration. The SESN3 antibody allows detailed analysis of these processes and helps map signaling interactions with FOXO and p53 transcription factors that drive stress-response gene expression. Dysregulation of Sestrin-3 has been implicated in tumor progression, where its expression may modulate cell survival under metabolic stress conditions common in cancer microenvironments.

The SESN3 antibody performs effectively in western blotting, immunohistochemistry, and immunofluorescence. It provides clear cytoplasmic staining consistent with the localization of this stress-inducible regulator. NSJ Bioreagents offers the SESN3 antibody as a validated reagent for reproducible detection across research platforms. By supporting detailed exploration of Sestrin-3 function in redox balance, energy metabolism, and autophagy, the SESN3 antibody advances understanding of cellular adaptation mechanisms that maintain metabolic health and longevity.

UniProt: P58005