Recombinant Topoisomerase II alpha antibody (AA 1300-1500)

Catalog No. ABIN7870497

The Mouse Monoclonal anti-Topoisomerase II alpha antibody is suitable to detect Topoisomerase II alpha in samples from Human. It has been validated for IHC (p).

Quick Overview for Recombinant Topoisomerase II alpha antibody (AA 1300-1500) (ABIN7870497)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: RTOP2A-8339

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1300-1500

Purpose: Recombinant TOP2A Antibody / Topoisomerase II alpha (azide and preservative free)

Purification: Protein A/G affinity

Immunogen: A recombinant human Topoisomerase II alpha protein fragment (aa1300-1500) was used as the immunogen for the recombinant TOP2A antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the recombinant TOP2A antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Aliquot the recombinant TOP2A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background: It recognizes a 170 kDa protein, which is identified as topoisomerase IIAlpha. It shows no cross-reaction with Topoisomerase II beta or I. Topo II Alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II Alpha is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases