Queuine tRNA-Ribosyltransferase Accessory Subunit 2 (QTRT2) (AA 142-386) antibody

Catalog No. ABIN7870690

The Rabbit Polyclonal anti-Queuine tRNA-Ribosyltransferase Accessory Subunit 2 antibody (ABIN7870690) specifically detects Queuine tRNA-Ribosyltransferase Accessory Subunit 2 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Queuine tRNA-Ribosyltransferase Accessory Subunit 2 (QTRT2) (AA 142-386) antibody (ABIN7870690)

Target: Queuine tRNA-Ribosyltransferase Accessory Subunit 2 (QTRT2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Product Details

Binding Specificity: AA 142-386

Purpose: QTRT2 Antibody / Queuine tRNA-ribosyltransferase subunit 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human QTRT2 recombinant protein (Position: Q142-H386) was used as the immunogen for the QTRT2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the QTRT2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the QTRT2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details

Target: Queuine tRNA-Ribosyltransferase Accessory Subunit 2 (QTRT2)

Alternative Name: QTRT2

Background: QTRT2 antibody detects Queuine tRNA-ribosyltransferase subunit 2, encoded by the QTRT2 gene on chromosome 6p25.1. QTRT2 antibody is widely used in RNA modification, translation, and metabolism research. QTRT2 partners with QTRT1 to form the heterodimeric tRNA-guanine transglycosylase complex that catalyzes incorporation of queuine into the anticodon wobble position of tRNAs. This modification enhances translation accuracy and efficiency.

Structurally, QTRT2 is a ~47 kDa protein that shares homology with QTRT1 but lacks catalytic residues. Instead, QTRT2 functions as a structural subunit that stabilizes the heterodimer and facilitates substrate recognition. QTRT2 localizes to the cytoplasm where tRNA modification occurs. It is conserved across eukaryotes, reflecting its fundamental role in translation regulation.

Functionally, QTRT2 supports tRNA modification that improves translational fidelity and codon-anticodon interactions. Queuine modification affects protein synthesis rates, cellular stress responses, and adaptation to nutrient availability. Researchers use QTRT2 antibody to investigate RNA modifications, translation quality control, and metabolic regulation.

Clinically, defects in queuine modification have been associated with cancer, neurodevelopmental disorders, and infection. Altered expression of QTRT2 has been reported in some tumors, suggesting a role in metabolic reprogramming and growth. Because RNA modifications are emerging therapeutic targets, QTRT2 is under active investigation. NSJ Bioreagents provides QTRT2 antibody to support RNA biology and translational control research.

Experimentally, QTRT2 antibody is applied in western blotting to detect the ~47 kDa protein, in immunofluorescence microscopy to study cytoplasmic localization, and in immunoprecipitation to analyze QTRT1-QTRT2 complexes. Functional assays combining QTRT2 antibody with RNA analysis confirm its role in tRNA modification.

UniProt: Q9H974