Recombinant Topoisomerase II alpha antibody (AA 1431-1531)

Catalog No. ABIN7870710

The Rabbit Monoclonal anti-Topoisomerase II alpha antibody is suitable to detect Topoisomerase II alpha in samples from Human and Dog. It has been validated for IHC (p).

Quick Overview for Recombinant Topoisomerase II alpha antibody (AA 1431-1531) (ABIN7870710)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Antibody Type: Recombinant Antibody

Reactivity: Human, Dog

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: TOP2A-7169R

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1431-1531

Purpose: Topoisomerase II alpha Antibody / TOP2A (azide and preservative free)

Purification: Protein A/G affinity

Immunogen: A recombinant partial protein sequence (within amino acids 1431-1531) from the human protein was used as the immunogen for the Topoisomerase II alpha antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the Topoisomerase II alpha antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Aliquot the Topoisomerase II alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: Topoisomerase II alpha

Background: The topoisomerase II-Alpha isoform is a 170 kDa nuclear protein and plays an important role in DNA synthesis and RNA transcription, as well as chromosomal segregation during mitosis. Topoisomerase II-Alpha is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells, and has been shown to be over-expressed in many human cancers. Decreased expression of Topoisomerase II-Alpha is the predominant mechanism of resistance to several chemotherapeutic agents.

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases