Recombinant Topoisomerase II alpha antibody (AA 1431-1531)

Catalog No. ABIN7870711

The Rabbit Monoclonal anti-Topoisomerase II alpha antibody (Clone TOP2A-7169R) (ABIN7870711) specifically detects Topoisomerase II alpha in IHC (p). The antibody is reactive with Human and Dog samples.

Quick Overview for Recombinant Topoisomerase II alpha antibody (AA 1431-1531) (ABIN7870711)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Antibody Type: Recombinant Antibody

Reactivity: Human, Dog

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TOP2A-7169R

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1431-1531

Purpose: Topoisomerase II alpha Antibody / TOP2A

Purification: Protein A/G affinity

Immunogen: A recombinant partial protein sequence (within amino acids 1431-1531) from the human protein was used as the immunogen for the Topoisomerase II alpha antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the Topoisomerase II alpha antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Topoisomerase II alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: Topoisomerase II alpha

Background: The topoisomerase II-Alpha isoform is a 170 kDa nuclear protein and plays an important role in DNA synthesis and RNA transcription, as well as chromosomal segregation during mitosis. Topoisomerase II-Alpha is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells, and has been shown to be over-expressed in many human cancers. Decreased expression of Topoisomerase II-Alpha is the predominant mechanism of resistance to several chemotherapeutic agents.

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases