BOP1 antibody (AA 157-701)

Catalog No. ABIN7871024

This Rabbit Polyclonal antibody specifically detects BOP1 in WB, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for BOP1 antibody (AA 157-701) (ABIN7871024)

Target: BOP1 (Block of Proliferation 1 (BOP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BOP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-BOP1 Antibody

Binding Specificity: AA 157-701

Purpose: BOP1 Antibody / Block of proliferation 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human BOP1 recombinant protein (Position: R157-N701) was used as the immunogen for the BOP1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the BOP1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the BOP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for BOP1

Target: BOP1 (Block of Proliferation 1 (BOP1))

Alternative Name: BOP1

Background: BOP1 antibody detects Block of proliferation 1 protein, a nucleolar factor that plays a central role in ribosome biogenesis, cell cycle regulation, and tumor progression. BOP1 forms part of the PeBoW complex (with PES1 and WDR12), which is required for maturation of the 28S and 5.8S ribosomal RNAs and assembly of the 60S ribosomal subunit. The BOP1 antibody is used in studies of ribosome formation, cellular proliferation, and oncogenic transformation.

BOP1 is encoded by the BOP1 gene located on human chromosome 8q24.3, a genomic region frequently amplified in cancers. The protein is approximately 80 kilodaltons and contains WD40 repeat motifs that mediate protein-protein interactions within the nucleolus. BOP1 functions as a structural scaffold coordinating pre-rRNA processing and ribonucleoprotein complex formation. Its proper activity is critical for balanced ribosome production and maintenance of genomic stability.

The BOP1 antibody detects a 100-110 kilodalton band by western blot and exhibits strong nucleolar localization by immunofluorescence. During ribosome assembly, BOP1 interacts with PES1 and WDR12 to stabilize preribosomal particles, ensuring efficient cleavage and processing of rRNA precursors. Loss of BOP1 results in accumulation of immature rRNA intermediates and nucleolar stress, leading to p53 activation and cell cycle arrest.

BOP1 also influences mitotic progression and centrosome duplication. Overexpression accelerates cell proliferation and promotes chromosomal instability, while depletion inhibits tumor cell growth. Dysregulation of BOP1 has been reported in colorectal, pancreatic, and hepatocellular carcinomas, correlating with poor prognosis and increased metastatic potential.

Because ribosome biogenesis is tightly linked to cell growth, BOP1 serves as a valuable biomarker for cancer diagnostics and as a potential target for anti-proliferative therapies. NSJ Bioreagents provides a validated BOP1 antibody optimized for western blot, immunofluorescence, and nucleolar imaging, supporting research into ribosomal RNA processing, nucleolar stress response, and oncogenic signaling pathways.

UniProt: Q14137