SUN5 antibody (AA 16-341)

Catalog No. ABIN7871069

This Rabbit Polyclonal antibody specifically detects SUN5 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SUN5 antibody (AA 16-341) (ABIN7871069)

Target: SUN5 (Sad1 and UNC84 Domain Containing 5 (SUN5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUN5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SUN5 Antibody

Binding Specificity: AA 16-341

Purpose: SUN5 Antibody / Sad1 and UNC84 domain-containing protein 5

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SUN5 recombinant protein (Position: E16-K341) was used as the immunogen for the SUN5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SUN5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SUN5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SUN5

Target: SUN5 (Sad1 and UNC84 Domain Containing 5 (SUN5))

Alternative Name: SUN5

Background: SUN5 antibody detects Sad1 and UNC84 domain-containing protein 5, a nuclear envelope protein that anchors the nucleus to the cytoskeleton during spermatogenesis. The UniProt recommended name is Sad1 and UNC84 domain-containing protein 5 (SUN5). This transmembrane protein is part of the linker of nucleoskeleton and cytoskeleton (LINC) complex, which connects nuclear components to cytoskeletal filaments, ensuring nuclear positioning and structural integrity.

Functionally, SUN5 antibody identifies a 379-amino-acid protein localized to the inner nuclear membrane, where it interacts with outer membrane proteins such as Nesprins through its conserved SUN domain. SUN5 is particularly enriched in spermatids, where it mediates head-to-tail coupling during sperm maturation. Loss of SUN5 disrupts this linkage, leading to acephalic spermatozoa and infertility.

The SUN5 gene is located on chromosome 20q11.22 and is predominantly expressed in testis, with lower expression in other tissues. Its testis-specific expression pattern underscores its specialized function in spermiogenesis. SUN5 contributes to nuclear shaping, centrosome attachment, and flagellar organization through mechanical coupling between the nucleus and cytoskeleton.

Pathologically, mutations in SUN5 are associated with acephalic spermatozoa syndrome and male infertility due to defective sperm head-tail junction formation. Research using SUN5 antibody supports studies in reproductive biology, nuclear envelope architecture, and cytoskeletal interaction mechanisms.

SUN5 antibody is validated for use in western blotting, immunofluorescence, and immunohistochemistry to study LINC complex components and spermatid structure. NSJ Bioreagents offers SUN5 antibody reagents optimized for cell biology and reproductive system research.

Structurally, SUN5 contains a coiled-coil region and a conserved C-terminal SUN domain that mediates interaction with KASH domain proteins across the nuclear envelope. This antibody helps elucidate SUN5's molecular function in nuclear-cytoskeletal attachment and fertility-related disorders.

UniProt: Q8TC36