NPTX2 antibody (AA 16-431)

Catalog No. ABIN7871079

The Rabbit Polyclonal anti-NPTX2 antibody is suitable to detect NPTX2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and ELISA.

Quick Overview for NPTX2 antibody (AA 16-431) (ABIN7871079)

Target: NPTX2 (Neuronal Pentraxin II (NPTX2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPTX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-NPTX2 Antibody

Binding Specificity: AA 16-431

Purpose: NPTX2 Antibody / Neuronal pentraxin 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human NPTX2 recombinant protein (Position: G16-L431) was used as the immunogen for the NPTX2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NPTX2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NPTX2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NPTX2

Target: NPTX2 (Neuronal Pentraxin II (NPTX2))

Alternative Name: NPTX2

Background: NPTX2 antibody detects Neuronal pentraxin-2, a secreted neuronal glycoprotein involved in synaptic plasticity, excitatory synapse formation, and long-term neuronal communication. NPTX2 belongs to the neuronal pentraxin family, which includes NPTX1 and NPTR, proteins that share structural similarities with classical acute-phase pentraxins but function primarily in the central nervous system. The NPTX2 antibody is widely used to study synapse organization, neuronal activity regulation, and neurodegenerative disorders such as Alzheimer's disease.

NPTX2 is encoded by the NPTX2 gene located on human chromosome 7q21.3. The protein is approximately 47 kilodaltons and secreted from presynaptic terminals, where it binds AMPA-type glutamate receptors on postsynaptic neurons. This interaction enhances receptor clustering, synaptic strength, and excitatory transmission. NPTX2 is highly expressed in cortical and hippocampal neurons and plays a key role in activity-dependent synaptic refinement during learning and memory.

The NPTX2 antibody detects a 47 kilodalton band on western blot. In the brain, NPTX2 is upregulated by neuronal activity and acts in concert with NPTX1 and NPTR to regulate synaptic homeostasis. Disruption of NPTX2 expression leads to impaired long-term potentiation and deficits in learning and behavior. Cerebrospinal fluid levels of NPTX2 have been proposed as a biomarker for early-stage neurodegeneration, with reductions correlating to cognitive decline in Alzheimer's and frontotemporal dementia.

At the molecular level, NPTX2 binds to neuronal pentraxin receptor (NPTXR) to form hetero-oligomeric complexes that tether AMPA receptors to the postsynaptic membrane. This complex promotes excitatory synapse stabilization and neurotransmitter signaling efficiency. Dysregulation of this system contributes to excitatory-inhibitory imbalance, a hallmark of several neuropsychiatric and neurodegenerative conditions.

Beyond the nervous system, NPTX2 expression has been observed in pancreatic islets and endocrine tissues, where it may regulate calcium-dependent secretion. NSJ Bioreagents provides a validated NPTX2 antibody optimized for western blot and immunohistochemistry, supporting research into neuronal plasticity, receptor clustering, and disease-related synaptic dysfunction.

UniProt: P47972