SF3A1 antibody (AA 16-646)

Catalog No. ABIN7871091

The Rabbit Polyclonal anti-SF3A1 antibody is suitable to detect SF3A1 in samples from Zebrafish (Danio rerio). It has been validated for IF and IHC (p).

Quick Overview for SF3A1 antibody (AA 16-646) (ABIN7871091)

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3A1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SF3A1 Antibody

Binding Specificity: AA 16-646

Purpose: Zebrafish Sf3a1 Antibody / Splicing factor 3A subunit 1

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Sf3a1 recombinant protein (amino acids K16-P646) was used as the immunogen for the Zebrafish Sf3a1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Sf3a1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Sf3a1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SF3A1

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Alternative Name: Sf3a1

Background: Zebrafish Sf3a1, also known as splicing factor 3A subunit 1, is a critical component of the spliceosome complex that regulates the removal of introns from pre messenger RNA. Sf3a1 is part of the SF3A protein complex, which works together with the SF3B complex to stabilize the binding of the U2 small nuclear ribonucleoprotein to the branch point sequence during splicing. This step is essential for accurate and efficient splicing, which in turn is vital for the generation of mature and functional messenger RNA transcripts.

Zebrafish Sf3a1 is an ortholog of the human SF3A1 protein and shares significant sequence and functional conservation. Like its human counterpart, zebrafish Sf3a1 is required for normal RNA processing and proper regulation of gene expression. Due to the importance of splicing in controlling developmental and cellular processes, Sf3a1 function is essential during embryogenesis and tissue differentiation.

Currently, there are no known isoforms of zebrafish Sf3a1 that have been characterized, but as with other splicing factors, alternative splicing may generate additional transcript variants that are yet to be fully studied. Research into zebrafish Sf3a1 offers valuable insights into conserved mechanisms of splicing regulation and its roles in development and disease.

Antibodies targeting zebrafish Sf3a1 protein are useful tools for examining spliceosomal assembly, RNA processing, and transcriptional regulation. These antibodies are commonly used in applications such as western blot, immunohistochemistry, immunofluorescence, and ELISA, supporting a broad range of molecular and developmental biology studies.

UniProt: Q90X41

Pathways: Ribonucleoprotein Complex Subunit Organization