Osteoactivin antibody (AA 164-564)

Catalog No. ABIN7871133

The Rabbit Polyclonal anti-Osteoactivin antibody is suitable to detect Osteoactivin in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, FACS, ICC and IF.

Quick Overview for Osteoactivin antibody (AA 164-564) (ABIN7871133)

Target: Osteoactivin (GPNMB)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteoactivin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Osteoactivin Antibody

Binding Specificity: AA 164-564

Purpose: GPNMB Antibody / Transmembrane glycoprotein NMB / HGFN

Purification: Immunogen affinity purified

Immunogen: E.coli-derived mouse GPNMB recombinant protein (Position: R164-D564) was used as the immunogen for the GPNMB antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GPNMB antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GPNMB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Osteoactivin

Target: Osteoactivin (GPNMB)

Alternative Name: GPNMB

Background: GPNMB antibody detects Transmembrane glycoprotein NMB, a type I membrane protein involved in cell adhesion, differentiation, and tissue repair. The UniProt recommended name is Transmembrane glycoprotein NMB (GPNMB). Also known as Osteoactivin or HGFN, this glycoprotein is expressed in osteoclasts, melanocytes, macrophages, and tumor cells, where it contributes to cell signaling and microenvironment remodeling.

Functionally, GPNMB antibody identifies a 560-amino-acid glycoprotein with an extracellular domain containing an RGD-like integrin-binding motif, a transmembrane helix, and a short cytoplasmic tail with a tyrosine-based sorting signal. GPNMB facilitates cell-matrix interaction and promotes osteoblast differentiation, pigment cell function, and immune modulation. It is also upregulated during tissue repair and inflammation, reflecting its role in regeneration and immune regulation.

The GPNMB gene is located on chromosome 7p15.2 and encodes a protein expressed in bone, skin, brain, and immune cells. In macrophages and dendritic cells, GPNMB modulates cytokine release and antigen presentation, while in osteoclasts it contributes to bone resorption and remodeling. The protein undergoes ectodomain shedding, releasing soluble fragments that influence neighboring cells.

Pathologically, GPNMB is overexpressed in several cancers, including melanoma, breast, and glioma, where it promotes migration, invasion, and angiogenesis. It also plays roles in neurodegenerative diseases and inflammation. Because of its surface localization, GPNMB serves as a potential therapeutic target and biomarker. Research using GPNMB antibody supports studies of tumor biology, osteogenesis, and immune signaling.

GPNMB antibody is suitable for western blotting, immunohistochemistry, and flow cytometry to detect membrane-associated and soluble GPNMB. NSJ Bioreagents offers validated GPNMB antibody reagents designed for research in cell adhesion, tumor biology, and tissue regeneration.

Structurally, Transmembrane glycoprotein NMB features multiple N-glycosylation sites contributing to stability and cell-surface expression. The cytoplasmic domain interacts with intracellular adaptors, linking extracellular signals to intracellular pathways. This antibody enables detection of GPNMB in studies of cell communication, repair, and disease progression.

UniProt: Q99P91