Hexokinase 1 antibody (AA 17-323)

Catalog No. ABIN7871223

The Mouse Monoclonal anti-Hexokinase 1 antibody is suitable to detect Hexokinase 1 in samples from Human, Mouse and Rat. It has been validated for WB, IF, IHC (p), FACS and IHC (fro).

Quick Overview for Hexokinase 1 antibody (AA 17-323) (ABIN7871223)

Target: Hexokinase 1 (HK1)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hexokinase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2I4

Product Details anti-Hexokinase 1 Antibody

Binding Specificity: AA 17-323

Purpose: HK1 Antibody / Hexokinase 1

Purification: Affinity purified

Immunogen: Recombinant human protein (amino acids D17-R323) was used as the immunogen for the HK1 antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the HK1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the HK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Hexokinase 1

Target: Hexokinase 1 (HK1)

Alternative Name: HK1

Background: Hexokinase-1 (HK1) is an enzyme that in humans is encoded by the HK1 gene on chromosome 10. It is mapped to 10q22.1. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.

UniProt: P19367

Pathways: Carbohydrate Homeostasis, Warburg Effect