Recombinant Vinculin antibody (AA 174-322)

Catalog No. ABIN7871291

This Rabbit Monoclonal antibody specifically detects Vinculin in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for Recombinant Vinculin antibody (AA 174-322) (ABIN7871291)

Target: Vinculin (VCL)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: VCL-7091R

Product Details anti-Vinculin Antibody

Binding Specificity: AA 174-322

Purpose: Recombinant Vinculin Antibody

Purification: Protein A/G affinity

Immunogen: A portion of amino acids 174-322 was used as the immunogen for the recombinant Vinculin antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the recombinant Vinculin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the recombinant Vinculin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin

Background: Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2