ASK1 antibody (AA 178-1112)

Catalog No. ABIN7871345

This Rabbit Polyclonal antibody specifically detects ASK1 in WB, ELISA, IHC (p) and FACS. It exhibits reactivity toward Human.

Quick Overview for ASK1 antibody (AA 178-1112) (ABIN7871345)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-ASK1 Antibody

Binding Specificity: AA 178-1112

Purpose: ASK1 Antibody / MAP3K5

Purification: Affinity purified

Immunogen: An E. coli-derived human protein (amino acids A178-K1112) was used as the immunogen for the ASK1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ASK1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ASK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: ASK1

Background: Apoptosis signal-regulating kinase 1 (ASK1) also known as mitogen-activated protein kinase 5 (MAP3K5) is a member of MAP kinase family and as such a part of mitogen-activated protein kinase pathway. Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.

UniProt: Q99683

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response