Recombinant Villin 1 antibody (AA 179-311)

Catalog No. ABIN7871357

This Rabbit Monoclonal antibody specifically detects Villin 1 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for Recombinant Villin 1 antibody (AA 179-311) (ABIN7871357)

Target: Villin 1 (VIL1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Villin 1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: VIL1-2310R

Product Details anti-Villin 1 Antibody

Binding Specificity: AA 179-311

Purpose: Recombinant Villin Antibody / VIL1

Purification: Protein A affinity chromatography

Immunogen: Amino acids 179-311 from the human protein were used as the immunogen for this recombinant Villin antibody.

Isotype: IgG, kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant Villin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Villin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Villin 1

Target: Villin 1 (VIL1)

Alternative Name: Villin

Background: Recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization