BIM antibody (AA 18-186)

Catalog No. ABIN7871398

The Rabbit Polyclonal anti-BIM antibody (ABIN7871398) specifically detects BIM in WB, ELISA and FACS. The antibody is reactive with Mouse samples.

Quick Overview for BIM antibody (AA 18-186) (ABIN7871398)

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BIM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-BIM Antibody

Binding Specificity: AA 18-186

Purpose: Bim Antibody / Bcl-2-like protein 11

Purification: Immunogen affinity purified

Immunogen: E.coli-derived mouse Bim/Bcl2l11 recombinant protein (Position: Q18-R186) was used as the immunogen for the Bim antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Bim antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Bim antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for BIM

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Alternative Name: Bim

Background: Bim antibody detects Bcl-2-like protein 11, a pro-apoptotic member of the Bcl-2 family that promotes programmed cell death through mitochondrial outer membrane permeabilization. The UniProt recommended name is Bcl-2-like protein 11 (BCL2L11). Also known as Bim, this BH3-only protein is a critical initiator of apoptosis in response to cellular stress, cytokine withdrawal, or DNA damage.

Functionally, Bim antibody identifies a 198-amino-acid cytoplasmic protein that binds anti-apoptotic Bcl-2 proteins (such as BCL2, BCL-XL, and MCL1), releasing pro-apoptotic effectors BAX and BAK to trigger mitochondrial cytochrome c release and caspase activation. Bim exists in several isoforms (BimEL, BimL, and BimS) generated by alternative splicing, which modulate apoptosis sensitivity across cell types.

The BCL2L11 gene is located on chromosome 2q13 and is expressed in lymphoid, neuronal, and epithelial tissues. Its expression is tightly regulated by survival pathways including PI3K/AKT and MAPK signaling, which control phosphorylation and proteasomal degradation of Bim.

Pathologically, dysregulation of Bim contributes to cancer, autoimmune diseases, and neurodegeneration. Reduced Bim expression confers resistance to apoptosis in tumor cells, while overexpression causes excessive neuronal death. Research using Bim antibody supports studies in cell death signaling, oncology, and stress-induced apoptosis.

Bim antibody is validated for western blotting, immunohistochemistry, and apoptosis assays to detect Bcl-2 family proteins. NSJ Bioreagents provides Bim antibody reagents optimized for apoptosis research, mitochondrial biology, and cancer cell signaling.

Structurally, Bcl-2-like protein 11 contains a BH3 domain required for binding to anti-apoptotic Bcl-2 members and activating the intrinsic apoptotic pathway. This antibody supports exploration of Bim's role in stress-induced mitochondrial apoptosis and cellular homeostasis.

UniProt: O54918

Pathways: PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity