RRN3 antibody (AA 184-651)

Catalog No. ABIN7871481

The Rabbit Polyclonal anti-RRN3 antibody is suitable to detect RRN3 in samples from Human. It has been validated for WB, ELISA and FACS.

Quick Overview for RRN3 antibody (AA 184-651) (ABIN7871481)

Target: RRN3 (RRN3 RNA Polymerase I Transcription Factor (RRN3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRN3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RRN3 Antibody

Binding Specificity: AA 184-651

Purpose: RRN3 Antibody / TIF-IA / RNA polymerase I-specific transcription initiation factor RRN3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human RRN3 recombinant protein (Position: D184-L651) was used as the immunogen for the RRN3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RRN3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RRN3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RRN3

Target: RRN3 (RRN3 RNA Polymerase I Transcription Factor (RRN3))

Alternative Name: RRN3

Background: RRN3 antibody detects RNA polymerase I-specific transcription initiation factor RRN3, encoded by the RRN3 gene on chromosome 16p13.11. RRN3 antibody is widely used in studies of transcriptional regulation, ribosome biogenesis, and cell growth. RRN3 is a transcription factor that links RNA polymerase I to the transcription machinery at ribosomal DNA (rDNA) promoters, initiating rRNA synthesis. This step is critical for ribosome production and cellular growth control.

Structurally, RRN3 is a ~65 kDa phosphoprotein that interacts with both RNA polymerase I and transcription initiation factors such as SL1. It contains multiple phosphorylation sites that regulate its activity and stability. Phosphorylation by mTOR and other kinases modulates its ability to recruit RNA polymerase I to rDNA promoters. RRN3 is localized to the nucleolus, consistent with its role in ribosome synthesis.

Functionally, RRN3 is essential for transcription initiation of rRNA genes. It bridges RNA polymerase I and the pre-initiation complex, enabling promoter recognition and transcriptional activation. Regulation of RRN3 activity controls ribosome biogenesis, cell cycle progression, and response to growth signals. Researchers use RRN3 antibody to investigate ribosomal transcription, nucleolar biology, and growth signaling pathways.

Clinically, dysregulation of RRN3 is linked to cancer, where elevated rRNA synthesis supports uncontrolled proliferation. mTOR-mediated regulation of RRN3 provides a mechanistic link between nutrient sensing and ribosome production. RRN3 expression changes have been observed in metabolic diseases and stress responses. Because ribosome biogenesis is a hallmark of cancer, RRN3 is a target for therapeutic intervention. NSJ Bioreagents offers RRN3 antibody for studies of transcription regulation, nucleolar activity, and oncogenesis.

Experimentally, RRN3 antibody is used in western blotting to detect the ~65 kDa protein, in immunofluorescence microscopy to analyze nucleolar localization, and in chromatin immunoprecipitation to study promoter recruitment. Co-immunoprecipitation with RRN3 antibody identifies interacting transcription factors and RNA polymerase I subunits.

UniProt: Q9NYV6

Pathways: Negative Regulation of intrinsic apoptotic Signaling