Fibromodulin antibody (AA 19-376)

Catalog No. ABIN7871589

The Rabbit Polyclonal anti-Fibromodulin antibody is suitable to detect Fibromodulin in samples from Human and Mouse. It has been validated for WB, ELISA and FACS.

Quick Overview for Fibromodulin antibody (AA 19-376) (ABIN7871589)

Target: Fibromodulin (FMOD)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibromodulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Fibromodulin Antibody

Binding Specificity: AA 19-376

Purpose: FMOD Antibody / Fibromodulin

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Fibromodulin/FMOD recombinant protein (Position: Q19-I376) was used as the immunogen for the FMOD antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the FMOD antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the FMOD antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Fibromodulin

Target: Fibromodulin (FMOD)

Alternative Name: FMOD

Background: FMOD antibody detects Fibromodulin, encoded by the FMOD gene. Fibromodulin is a small leucine-rich repeat proteoglycan of the extracellular matrix that regulates collagen fibrillogenesis and tissue organization. FMOD antibody provides researchers with a specific reagent to study extracellular matrix biology, connective tissue remodeling, and disease processes involving abnormal matrix composition.

Fibromodulin is secreted by fibroblasts and chondrocytes and associates with collagen fibrils. Research using FMOD antibody has shown that it binds to type I and type II collagen fibrils, regulating their diameter and spacing. This interaction ensures proper biomechanical properties of tissues such as cartilage, tendons, and skin. Without Fibromodulin, collagen fibrils are irregular in size and arrangement, leading to impaired tissue strength and function.

Studies with FMOD antibody have revealed roles in tissue repair and wound healing. Fibromodulin is upregulated following injury and promotes scar formation by modulating collagen deposition and organization. It also influences the recruitment of macrophages and fibroblasts, linking extracellular matrix remodeling with immune responses. These findings highlight the importance of Fibromodulin in regenerative biology.

Fibromodulin is also involved in angiogenesis. Research using FMOD antibody has demonstrated that it binds to growth factors and modulates endothelial cell function. By interacting with transforming growth factor beta and vascular endothelial growth factor pathways, Fibromodulin regulates angiogenic responses during development and repair. Dysregulation can contribute to impaired healing or pathological angiogenesis in tumors.

Disease studies have connected Fibromodulin with osteoarthritis, fibrosis, and cancer. Research using FMOD antibody has shown that altered Fibromodulin expression affects cartilage integrity and progression of degenerative joint disease. Elevated expression has been associated with fibrotic disorders, while aberrant expression in tumors may contribute to cancer progression by modifying extracellular matrix stiffness and promoting invasion.

FMOD antibody is widely applied in immunohistochemistry, western blotting, and immunofluorescence. Immunohistochemistry identifies expression in connective tissues, western blotting quantifies protein levels and isoforms, and immunofluorescence reveals localization within matrix-rich environments. These methods make FMOD antibody valuable in extracellular matrix research.

By supplying validated FMOD antibody reagents, NSJ Bioreagents supports studies into collagen biology, tissue repair, and disease. Detection of Fibromodulin provides insight into how small leucine-rich proteoglycans regulate extracellular matrix function and pathology.

UniProt: Q06828

Pathways: Glycosaminoglycan Metabolic Process