MED12 antibody (AA 1991-2025)

Catalog No. ABIN7871758

The Rabbit Polyclonal anti-MED12 antibody (ABIN7871758) specifically detects MED12 in WB and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for MED12 antibody (AA 1991-2025) (ABIN7871758)

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MED12 Antibody

Binding Specificity: AA 1991-2025

Purpose: Med12 Antibody

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 1991-2025 from the mouse protein was used as the immunogen for the Med12 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Med12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Med12 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for MED12

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Alternative Name: Med12

Background: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).

UniProt: A2AGH6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Tube Formation