ANO1 antibody (AA 2-101)

Catalog No. ABIN7871759

This Mouse Monoclonal antibody specifically detects ANO1 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for ANO1 antibody (AA 2-101) (ABIN7871759)

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ANO1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: DG1-1486

Product Details anti-ANO1 Antibody

Binding Specificity: AA 2-101

Purpose: DOG1 Antibody

Purification: Protein G affinity chromatography

Immunogen: A recombinant human protein corresponding to amino acids 2-101 of human DOG1 was used as the immunogen for this DOG1 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG1 antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the DOG1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Alternative Name: DOG1

Background: Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GIST s), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GIST s, including all c-kit negative GIST s. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GIST s is nearly identical: 94.4 % vs. 94.7 %.

UniProt: Q5XXA6