DDIT4 antibody (AA 20-49)

Catalog No. ABIN7871875

The Rabbit Polyclonal anti-DDIT4 antibody (ABIN7871875) specifically detects DDIT4 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for DDIT4 antibody (AA 20-49) (ABIN7871875)

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDIT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DDIT4 Antibody

Binding Specificity: AA 20-49

Purpose: REDD-1 Antibody / DDIT4

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 20-49 from the human protein was used as the immunogen for the REDD-1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the REDD-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the REDD-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for DDIT4

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Alternative Name: REDD-1

Background: REDD-1 is a novel transcriptional target of p53 induced following DNA damage. During embryogenesis, REDD-1 expression mirrors the tissue-specific pattern of the p53 family member p63, and TP63 null embryos show virtually no expression of REDD-1, which is restored in mouse embryo fibroblasts following p63 expression. In differentiating primary keratinocytes, TP63 and REDD-1 expression are coordinately downregulated, and ectopic expression of either gene inhibits in vitro differentiation. REDD1 appears to function in the regulation of reactive oxygen species (ROS), TP63 null fibroblasts have decreased ROS levels and reduced sensitivity to oxidative stress, which are both increased following ectopic expression of either TP63 or REDD-1. Thus, REDD-1 encodes a shared transcriptional target that implicates ROS in the p53-dependent DNA damage response and in p63-mediated regulation of epithelial differentiation.

UniProt: Q9NX09

Pathways: Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process