BRCA2 antibody (AA 200-500)

Catalog No. ABIN7872070

The Mouse Monoclonal anti-BRCA2 antibody (Clone BRCA2-2158) (ABIN7872070) specifically detects BRCA2 in IHC (p). The antibody is reactive with Human samples.

Quick Overview for BRCA2 antibody (AA 200-500) (ABIN7872070)

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRCA2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: BRCA2-2158

Product Details anti-BRCA2 Antibody

Binding Specificity: AA 200-500

Purpose: BRCA2 Antibody

Purification: Protein A/G affinity

Immunogen: A recombinant partial protein sequence (within amino acids 200-500) from the human protein was used as the immunogen for the BRCA2 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the BRCA2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the BRCA2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for BRCA2

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Alternative Name: BRCA2

Background: In 1990, a breast cancer susceptibility gene designated BRCA1 was localized to chromosome 17q. Mutations within this gene are believed to account for approximately 45 % of families with high incidence of breast cancer and at least 80 % of families with increased incidence of both early-onset breast cancer and ovarian cancer. A second breast cancer susceptibility gene, BRCA2 (breast cancer 2, early onset), located on chromosome 13q13.1, also confers a high incidence of breast cancer but, unlike BRCA1, does not confer a substantially elevated risk of ovarian cancer. Both BRCA1 and BRCA2 play a role in the maintainance of genome stability, particularly in the homologous recombination pathway for double-strand DNA repair. BRCA2 is regarded as a tumor suppressor gene, tumors with BRCA2 mutations exhibit loss of heterozygosity (LOH) of the wildtype allele. The protein encoded by the BRCA2 gene contains multiple copies of a 70 amino acid motif called the BRC motif. These motifs effect binding to the Rad51 recombinase, which operates in DNA repair.

UniProt: P51587

Pathways: DNA Damage Repair, M Phase, Maintenance of Protein Location