MCL-1 antibody (pSer159, pThr163)

Catalog No. ABIN743738

The Rabbit Polyclonal anti-MCL-1 antibody has been validated for WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect MCL-1 in samples from Human and Rat. There is 1 publication available.

Quick Overview for MCL-1 antibody (pSer159, pThr163) (ABIN743738)

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCL-1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MCL-1 Antibody

Binding Specificity: pSer159, pThr163

Specificity: These phosphorylation sites are homologous to Ser140 + Thr144 in Mouse and Ser139 + Thr143 in Rat.

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Mcl 1 around the phosphorylation site of Ser159/Thr163

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-MCL-1 antibody (ABIN743738)

Braig, Wiedmann, Liebl, Singer, Kubisch, Schreiner, Abhari, Wagner, Kazmaier, Fulda, Vollmar: "Pretubulysin: a new option for the treatment of metastatic cancer." in: Cell death & disease, Vol. 5, pp. e1001, (2014) (PubMed).

Target Details for MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Alternative Name: Mcl1 +

Background:

Synonyms: TM, EAT, MCL1L, MCL1S, Mcl-1, BCL2L3, MCL1-ES, bcl2-L-3, mcl1/EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, MCL1

Background: Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

Gene ID: 4170

UniProt: Q07820

Pathways: MAPK Signaling