NKD1 antibody (AA 21-368)

Catalog No. ABIN7872170

This Rabbit Polyclonal antibody specifically detects NKD1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for NKD1 antibody (AA 21-368) (ABIN7872170)

Target: NKD1 (Naked Cuticle Homolog 1 (NKD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NKD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NKD1 Antibody

Binding Specificity: AA 21-368

Purpose: NKD1 Antibody / Naked cuticle 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human NKD1 recombinant protein (Position: D21-K368) was used as the immunogen for the NKD1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NKD1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NKD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NKD1

Target: NKD1 (Naked Cuticle Homolog 1 (NKD1))

Alternative Name: NKD1

Background: NKD1 antibody detects Naked cuticle homolog 1, a cytoplasmic protein that negatively regulates Wnt signaling by antagonizing Dishevelled-mediated pathway activation. The UniProt recommended name is Naked cuticle homolog 1 (NKD1). This protein acts as a feedback inhibitor within the canonical Wnt/Beta-catenin signaling cascade, ensuring controlled gene transcription during embryogenesis, cell proliferation, and tissue differentiation.

Functionally, NKD1 antibody identifies a 471-amino-acid protein localized in the cytoplasm that binds to Dishevelled (DVL) proteins, sequestering them away from the plasma membrane and preventing downstream beta-catenin stabilization. By limiting beta-catenin nuclear translocation, NKD1 represses Wnt target gene expression, balancing cell fate decisions and tissue patterning. NKD1 expression itself is induced by Wnt signaling, creating a negative feedback loop essential for maintaining signaling homeostasis.

The NKD1 gene is located on chromosome 16q12.1 and encodes a conserved protein containing an EF-hand-like calcium-binding motif and a Dishevelled-binding region. It functions in developmental processes such as neural tube formation, axis specification, and organogenesis. In adult tissues, NKD1 continues to modulate Wnt activity to maintain epithelial integrity and stem cell renewal. Loss or silencing of NKD1 leads to excessive Wnt pathway activation, contributing to oncogenesis and abnormal cell proliferation.

In cancer research, NKD1 acts as a tumor suppressor. Reduced NKD1 expression has been observed in colorectal, gastric, and hepatocellular carcinomas, where Wnt hyperactivation drives tumor progression. Restoration of NKD1 expression inhibits beta-catenin accumulation and reduces metastatic potential, highlighting its role as a critical checkpoint in Wnt pathway regulation. Beyond oncology, NKD1 also influences bone formation, neurogenesis, and intestinal regeneration through its modulation of Wnt and planar cell polarity signaling.

NKD1 antibody is widely used in developmental biology, oncology, and cell signaling research. It is suitable for immunoblotting, immunofluorescence, and co-immunoprecipitation to investigate NKD1 expression, localization, and interaction with Wnt signaling components. Detection of NKD1 provides insight into Wnt feedback regulation and its implications in stem cell biology and cancer. In tissue models, NKD1 serves as a molecular marker for Wnt pathway inhibition and differentiation status.

Structurally, NKD1 contains conserved motifs that enable direct binding to DVL proteins and membranes enriched in phosphatidylinositol phosphates. Its function is regulated by phosphorylation and intracellular calcium levels. NSJ Bioreagents provides NKD1 antibody reagents validated for use in Wnt signaling, developmental biology, and cancer pathway research.

UniProt: Q969G9