MLKL antibody (AA 210-464)

Catalog No. ABIN7872184

The Rabbit Polyclonal anti-MLKL antibody (ABIN7872184) specifically detects MLKL in WB, ELISA, IF, IHC (p) and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MLKL antibody (AA 210-464) (ABIN7872184)

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLKL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-MLKL Antibody

Binding Specificity: AA 210-464

Purpose: MLKL Antibody

Purification: Antigen affinity chromatography

Immunogen: An E.coli-derived human recombinant protein (amino acids R210-K464) was used as the immunogen for the MLKL antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MLKL antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MLKL Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MLKL

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Alternative Name: MLKL

Background: Mixed lineage kinase domain like pseudokinase (MLKL) is a protein that in humans is encoded by the MLKL gene. This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain, however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.

UniProt: Q8NB16