PPP1R15B antibody (AA 211-657)

Catalog No. ABIN7872192

The Rabbit Polyclonal anti-PPP1R15B antibody is suitable to detect PPP1R15B in samples from Human. It has been validated for WB, ELISA, IF and FACS.

Quick Overview for PPP1R15B antibody (AA 211-657) (ABIN7872192)

Target: PPP1R15B (Protein Phosphatase 1, Regulatory Subunit 15B (PPP1R15B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R15B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-PPP1R15B Antibody

Binding Specificity: AA 211-657

Purpose: PPP1R15B Antibody / Protein phosphatase 1 regulatory subunit 15B

Purification: Antigen affinity purified

Immunogen: E. coli-derived recombinant human protein (amino acids Q211-D657) was used as the immunogen for the PPP1R15B antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PPP1R15B antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PPP1R15B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PPP1R15B

Target: PPP1R15B (Protein Phosphatase 1, Regulatory Subunit 15B (PPP1R15B))

Alternative Name: PPP1R15B

Background: PPP1R15B (Protein phosphatase 1, regulatory subunit 15b), also called CREP, promotes dephosphorylation of the transcription initiation factor EIF2-alpha through recruitment of protein phosphatase-1(PP1) catalytic subunits. The PPP1R15B gene is mapped to chromosome 1q32.1 based on an alignment of thePPP1R15B sequence by Hartz(2010). Harding et al.(2009) obtained Ppp1r15b -/- mice at a mendelian ratio. However, Ppp1r15b -/- newborns were half the size of their wildtype littermates, were notably pale, and failed to nurse, and none survived the first day of postnatal life. Ppp1r15b -/- embryos that were also homozygous for an Eif2-alpha mutation that prevented Eif2-alpha phosphorylation were normalized, including elevated birth size and restored red blood cell count, compared with Ppp1r15b -/- embryos with wildtype Eif2-alpha.

UniProt: Q5SWA1

Pathways: ER-Nucleus Signaling