MLF1 antibody (AA 215-244)

Catalog No. ABIN7872230

This Rabbit Polyclonal antibody specifically detects MLF1 in WB, FACS and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for MLF1 antibody (AA 215-244) (ABIN7872230)

Target: MLF1 (Myeloid Leukemia Factor 1 (MLF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLF1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MLF1 Antibody

Binding Specificity: AA 215-244

Purpose: MLF1 Antibody / Myeloid leukemia factor 1

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 215-244 from the human protein was used as the immunogen for the MLF1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the MLF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MLF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for MLF1

Target: MLF1 (Myeloid Leukemia Factor 1 (MLF1))

Alternative Name: MLF1

Background: MLF1 is involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. The protein interferes with erythopoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. It suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. It binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.

UniProt: P58340