PIK3C2A antibody (AA 22-1523)

Catalog No. ABIN7872277

The Rabbit Polyclonal anti-PIK3C2A antibody (ABIN7872277) specifically detects PIK3C2A in WB, ELISA, ICC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for PIK3C2A antibody (AA 22-1523) (ABIN7872277)

Target: PIK3C2A (Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3C2A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-PIK3C2A Antibody

Binding Specificity: AA 22-1523

Purpose: PIK3C2A Antibody / Phosphoinositide 3-kinase-C2-alpha

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PIK3C2A recombinant protein (Position: R22-E1523) was used as the immunogen for the PIK3C2A antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PIK3C2A antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PIK3C2A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PIK3C2A

Target: PIK3C2A (Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A))

Alternative Name: PIK3C2A

Background: PIK3C2A antibody detects Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing alpha polypeptide, a lipid kinase that regulates membrane trafficking, signal transduction, and cytoskeletal organization. Encoded by the PIK3C2A gene on chromosome 11p15.5, this enzyme belongs to the class II phosphoinositide 3-kinase (PI3K) family and phosphorylates phosphatidylinositol and phosphatidylinositol 4-phosphate to generate phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2). Through these lipid intermediates, PIK3C2A coordinates endocytosis, vesicle trafficking, and cell polarity regulation.

PIK3C2A contains a catalytic kinase domain, a C2 lipid-binding domain, and clathrin-binding regions that localize it to the plasma membrane and endosomes. Its enzymatic activity is essential for clathrin-mediated endocytosis and autophagosome formation. PIK3C2A also modulates signaling pathways downstream of receptor tyrosine kinases and integrins by influencing AKT activation and cytoskeletal reorganization.

The PIK3C2A antibody is widely used in cell signaling, membrane biology, and cancer research to study phosphoinositide metabolism, vesicle formation, and kinase regulation. Western blot analysis identifies a 180-190 kilodalton band corresponding to PIK3C2A, while immunofluorescence reveals punctate staining in endosomal and plasma membrane compartments. This antibody enables the analysis of lipid signaling networks that control intracellular transport and cellular morphology.

Loss or mutation of PIK3C2A has been linked to congenital cataracts, vascular defects, and impaired cilia formation due to disrupted membrane trafficking. Conversely, overexpression contributes to tumorigenesis through altered PI3K signaling and enhanced migration. The PIK3C2A antibody provides a robust tool for investigating lipid kinase function in physiology and disease. NSJ Bioreagents validates this antibody for western blotting and immunofluorescence, ensuring accuracy for research into phosphoinositide and trafficking pathways.

UniProt: O00443

Pathways: EGFR Signaling Pathway, Inositol Metabolic Process, Platelet-derived growth Factor Receptor Signaling