DCTPP1 antibody (AA 22-170)

Catalog No. ABIN7872285

The Rabbit Polyclonal anti-DCTPP1 antibody is suitable to detect DCTPP1 in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and FACS.

Quick Overview for DCTPP1 antibody (AA 22-170) (ABIN7872285)

Target: DCTPP1 (DCTP Pyrophosphatase 1 (DCTPP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCTPP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-DCTPP1 Antibody

Binding Specificity: AA 22-170

Purpose: DCTPP1 Antibody / dCTP pyrophosphatase 1 / XTP3TPA

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human XTP3TPA/DCTPP1 recombinant protein (Position: R22-T170) was used as the immunogen for the DCTPP1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the DCTPP1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the DCTPP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for DCTPP1

Target: DCTPP1 (DCTP Pyrophosphatase 1 (DCTPP1))

Alternative Name: DCTPP1

Background: DCTPP1 antibody detects dCTP pyrophosphatase 1 (also known as XTP3TPA), a nucleotide pool sanitizing enzyme that prevents the incorporation of damaged or modified nucleotides into DNA. Encoded by the DCTPP1 gene on chromosome 16p13.3, this enzyme hydrolyzes dCTP and its oxidized derivatives to their monophosphate forms, maintaining genomic integrity by reducing mutagenic nucleotide incorporation. DCTPP1 plays an essential role in DNA replication fidelity, repair, and protection against oxidative stress-induced mutagenesis.

Located primarily in the nucleus and mitochondria, DCTPP1 acts as a homotetrameric hydrolase with substrate specificity for dCTP and 5-methyl-dCTP. By regulating the deoxynucleotide pool, it prevents mispairing and epigenetic instability caused by cytosine modifications. Its activity supports accurate DNA replication and limits the formation of base substitution mutations. DCTPP1 expression increases under oxidative stress and in proliferating cells to safeguard replication accuracy.

The DCTPP1 antibody is widely used in DNA metabolism, cancer, and oxidative stress research to investigate nucleotide sanitization, genome maintenance, and replication control. Western blot analysis detects a 21 kilodalton band corresponding to DCTPP1, while immunofluorescence reveals both nuclear and mitochondrial localization depending on cell type. This antibody enables researchers to explore DNA repair dynamics and nucleotide pool balance in diverse biological systems.

Loss or dysregulation of DCTPP1 is associated with increased mutation rates, mitochondrial dysfunction, and genomic instability. Elevated expression has been observed in rapidly dividing tumor cells, where it supports proliferation by maintaining balanced nucleotide pools. The DCTPP1 antibody provides a powerful tool for studying DNA replication accuracy, oxidative defense, and cancer cell metabolism. NSJ Bioreagents supplies this antibody validated for its applications, ensuring consistent detection in studies of genome integrity and nucleotide metabolism.

UniProt: Q9H773

Pathways: SARS-CoV-2 Protein Interactome