TWSG1 antibody (AA 22-199)

Catalog No. ABIN7872289

The Rabbit Polyclonal anti-TWSG1 antibody (ABIN7872289) specifically detects TWSG1 in WB, IHC, ELISA, FACS and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TWSG1 antibody (AA 22-199) (ABIN7872289)

Target: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TWSG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-TWSG1 Antibody

Binding Specificity: AA 22-199

Purpose: TWSG1 Antibody / Twisted gastrulation protein homolog 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TWSG1 recombinant protein (Position: E22-A199) was used as the immunogen for the TWSG1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TWSG1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TWSG1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TWSG1

Target: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Alternative Name: TWSG1

Background: TWSG1 antibody detects Twisted gastrulation protein homolog 1, a secreted modulator of bone morphogenetic protein (BMP) signaling that plays essential roles in embryonic development, tissue morphogenesis, and extracellular matrix regulation. The UniProt recommended name is Twisted gastrulation protein homolog 1 (TWSG1). This glycoprotein interacts with BMP ligands and antagonists to fine-tune morphogen gradients during organogenesis and cellular differentiation.

Functionally, TWSG1 antibody identifies a 223-amino-acid secreted protein that forms complexes with Chordin and BMPs (including BMP2, BMP4, and BMP7), modulating the bioavailability and receptor binding of BMP ligands. TWSG1 can either inhibit or enhance BMP signaling depending on tissue context, concentration, and association with proteases such as tolloid metalloproteinases. It contributes to dorsoventral patterning, skeletal formation, and hematopoietic stem cell maintenance by maintaining optimal BMP activity levels during development.

The TWSG1 gene is located on chromosome 18p11.21 and is expressed in a variety of embryonic and adult tissues including brain, kidney, bone, and reproductive organs. Expression is dynamically regulated by growth factors, Wnt signaling, and retinoic acid, ensuring coordinated morphogenic signaling across developing tissues.

Pathologically, mutations or reduced expression of TWSG1 cause craniofacial malformations and developmental defects due to disrupted BMP gradients. Altered TWSG1 signaling has also been implicated in cancer, fibrosis, and abnormal bone remodeling. Overexpression may contribute to tumor progression by influencing extracellular matrix deposition and epithelial-mesenchymal transition. Research using TWSG1 antibody supports studies in developmental biology, morphogen signaling, and cancer research.

TWSG1 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect secreted morphogen modulators. NSJ Bioreagents provides TWSG1 antibody reagents optimized for studies in BMP signaling, embryogenesis, and tissue differentiation.

Structurally, Twisted gastrulation protein homolog 1 contains two cysteine-rich domains stabilized by disulfide bonds, essential for ligand binding and signaling modulation. These domains enable interactions with both BMPs and their inhibitors, allowing TWSG1 to function as a molecular switch in morphogen networks. This antibody enables exploration of TWSG1's role in extracellular signaling, organ patterning, and skeletal formation.

UniProt: Q9GZX9