NCF1 antibody (AA 22-384)

Catalog No. ABIN7872322

The Rabbit Polyclonal anti-NCF1 antibody (ABIN7872322) specifically detects NCF1 in WB, IHC, ELISA, IF and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NCF1 antibody (AA 22-384) (ABIN7872322)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-NCF1 Antibody

Binding Specificity: AA 22-384

Purpose: NCF1 Antibody / Neutrophil cytosolic factor 1 / p47phox

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human p47 phox/NCF1 recombinant protein (Position: Q22-R384) was used as the immunogen for the NCF1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NCF1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NCF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Alternative Name: NCF1

Background: The NCF1 antibody targets Neutrophil cytosolic factor 1, a cytoplasmic component of the NADPH oxidase complex responsible for generating reactive oxygen species (ROS) during immune responses. Encoded by the NCF1 gene, this protein, also known as p47phox, acts as an organizer subunit that bridges cytosolic oxidase components with the membrane-bound catalytic core. The NCF1 antibody is a vital reagent for understanding how oxidative bursts are initiated in phagocytes and how these processes regulate host defense, inflammation, and redox signaling.

Neutrophil cytosolic factor 1 becomes phosphorylated upon cellular activation, prompting its translocation to the plasma membrane where it associates with p22phox, gp91phox (CYBB), and other subunits to assemble the active NADPH oxidase complex. This enzymatic system produces superoxide radicals essential for microbial killing. The NCF1 antibody enables detection of this phosphorylation-dependent activation and helps identify conditions that modulate oxidase assembly.

Genetic defects in NCF1 lead to chronic granulomatous disease (CGD), a primary immunodeficiency characterized by recurrent infections due to defective superoxide generation. In such patients, mutations often cause reduced or absent p47phox expression. Using the NCF1 antibody, researchers can distinguish functional versus nonfunctional protein forms, aiding in diagnostic and mechanistic studies of CGD. Beyond innate immunity, Neutrophil cytosolic factor 1 also contributes to redox-dependent signaling in endothelial and neuronal cells, highlighting its importance beyond phagocytes.

The NCF1 antibody is suitable for western blotting, immunoprecipitation, and flow cytometry, where it detects both total and phosphorylated protein forms. Its applications extend to studies examining oxidative stress, cell signaling cascades, and inflammatory pathway regulation. Because NADPH oxidase activity affects vascular tone, apoptosis, and cytokine production, NCF1 has broad physiological relevance. The antibody provides a reliable means to quantify expression changes under oxidative or inflammatory stress.

In oncology and cardiovascular research, aberrant activation of NCF1-containing complexes has been associated with chronic inflammation and tissue damage. Elevated expression may amplify ROS production, promoting DNA damage and tumorigenesis. Conversely, impaired function can weaken immune defense and tissue repair. The NCF1 antibody offered by NSJ Bioreagents supports detailed exploration of these opposing roles, helping define how redox balance influences health and disease.

By providing consistent detection across multiple platforms, the NCF1 antibody remains an essential reagent for studying oxidative metabolism and immune cell function. Its use continues to expand from immunology into neurobiology and cancer biology, where understanding reactive oxygen mechanisms is increasingly central to therapeutic innovation.

UniProt: P14598

Pathways: PI3K-Akt Signaling