Ki-67 antibody (AA 2293-2478)

Catalog No. ABIN7872449

This Mouse Monoclonal antibody specifically detects Ki-67 in FACS, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Ki-67 antibody (AA 2293-2478) (ABIN7872449)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PMKI67-1

Product Details anti-Ki-67 Antibody

Binding Specificity: AA 2293-2478

Purpose: Ki67 Antibody

Purification: Protein G affinity chromatography

Immunogen: A portion of amino acids 2293-2478 was used as the immunogen for the Ki67 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Ki67 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Ki67 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki67

Background: Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. [UniProt]

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process