KIAA0090 antibody (AA 23-993)

Catalog No. ABIN7872559

The Rabbit Polyclonal anti-KIAA0090 antibody (ABIN7872559) specifically detects KIAA0090 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for KIAA0090 antibody (AA 23-993) (ABIN7872559)

Target: KIAA0090

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIAA0090 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-KIAA0090 Antibody

Binding Specificity: AA 23-993

Purpose: EMC1 Antibody / ER membrane protein complex subunit 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human KIAA0090/EMC1 recombinant protein (Position: Y23-R993) was used as the immunogen for the EMC1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the EMC1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the EMC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for KIAA0090

Target: KIAA0090

Alternative Name: EMC1

Background: EMC1 antibody detects ER membrane protein complex subunit 1, a structural component of the ER membrane protein complex (EMC) responsible for folding and insertion of transmembrane proteins. EMC1 acts as a scaffold stabilizing the entire complex and supports proper biogenesis of multipass membrane proteins. The EMC1 antibody is used in cell biology, proteostasis, and neurodevelopmental research to study membrane protein maturation and ER quality control.

EMC1 is encoded by the EMC1 gene located on human chromosome 1p36.13. The protein is approximately 993 amino acids long and localizes to the endoplasmic reticulum membrane, where it forms part of a 10-subunit complex including EMC2 through EMC10. EMC1 contains multiple transmembrane segments and luminal domains that interact with nascent polypeptides emerging from the ribosome-translocon system.

The EMC1 antibody detects a 115 kilodalton band by western blot and shows reticular ER staining under immunofluorescence microscopy. EMC1 facilitates the co-translational insertion and folding of membrane proteins, including G-protein coupled receptors, ion channels, and transporters. It also interacts with the ER-associated degradation (ERAD) machinery to remove misfolded proteins and prevent aggregation.

Mutations in EMC1 lead to congenital disorders involving neurodevelopmental delay, cerebellar atrophy, and visual impairment, highlighting its importance in neuronal function. In model systems, EMC1 loss disrupts ER morphology and mitochondrial contacts, leading to stress responses and apoptosis. EMC1 also contributes to cholesterol biosynthesis by ensuring proper insertion of enzymes involved in lipid metabolism.

Through its essential role in membrane protein biogenesis and ER stability, EMC1 is a cornerstone of cellular proteostasis. NSJ Bioreagents provides a validated EMC1 antibody optimized for its applications, supporting studies of protein folding, ER homeostasis, and disease-associated misfolding mechanisms.

UniProt: Q8N766

Pathways: SARS-CoV-2 Protein Interactome