MMP12 antibody (AA 237-442)

Catalog No. ABIN7872637

The Rabbit Polyclonal anti-MMP12 antibody (ABIN7872637) specifically detects MMP12 in WB and ELISA. The antibody is reactive with Mouse samples.

Quick Overview for MMP12 antibody (AA 237-442) (ABIN7872637)

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MMP12 Antibody

Binding Specificity: AA 237-442

Purpose: Mmp12 Antibody / Matrix metalloproteinase 12

Purification: Immunogen affinity purified

Immunogen: E.coli-derived mouse Mmp12 recombinant protein (Position: K237-H442) was used as the immunogen for the Mmp12 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Mmp12 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Mmp12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MMP12

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Alternative Name: Mmp12

Background: MMP12 antibody detects Matrix metalloproteinase-12, also known as macrophage metalloelastase, an extracellular matrix-degrading enzyme that plays a key role in tissue remodeling, inflammation, and innate immune defense. The UniProt recommended name is Matrix metalloproteinase-12 (MMP12), and alternate names include macrophage metalloelastase, hME, macrophage elastase, and MME. MMP12 belongs to the matrix metalloproteinase (MMP) family, a group of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and regulate numerous physiological and pathological processes including wound healing, angiogenesis, and tumor progression.

MMP12 is predominantly expressed in macrophages, where it contributes to tissue turnover and inflammatory regulation by cleaving elastin, fibronectin, laminin, and type IV collagen. MMP12 antibody is often used in immunohistochemistry, western blot, and zymography to assess macrophage activation and ECM degradation activity. During chronic inflammation, such as in atherosclerosis, emphysema, and rheumatoid arthritis, overproduction of MMP12 accelerates ECM breakdown and tissue destruction. Conversely, controlled MMP12 activity is necessary for effective wound repair, pathogen clearance, and extracellular remodeling in normal immune responses.

Structurally, MMP12 contains the conserved catalytic zinc-binding motif (HEXXHXXGXXH) and a C-terminal hemopexin domain important for substrate specificity and interaction with tissue inhibitors of metalloproteinases (TIMPs). The MMP12 antibody is used to monitor expression and activity under various conditions where macrophage-mediated proteolysis contributes to disease pathology. The MMP12 gene is located on chromosome 11q22.2-q22.3, clustered with other MMP family members including MMP1, MMP3, and MMP13. The mature enzyme is secreted as a pro-MMP, which is activated by proteolytic cleavage or oxidative stress in inflamed tissues.

Beyond matrix degradation, MMP12 regulates cytokine bioavailability and macrophage migration, influencing the balance between inflammation and resolution. Elevated MMP12 levels have been correlated with chronic obstructive pulmonary disease (COPD) severity and vascular remodeling in atheromatous plaques. Genetic knockout models in mice have shown Mmp12 deletion reduces elastin breakdown and lung injury, highlighting its central role in tissue integrity. Research using MMP12 antibody supports studies of inflammation, fibrosis, tumor invasion, and innate immunity. NSJ Bioreagents provides validated reagents that detect human, mouse, and rat MMP12 in tissues and culture systems for translational and mechanistic research.

UniProt: P34960