N-Cadherin antibody (AA 25-810)

Catalog No. ABIN7872893

This Rabbit Polyclonal antibody specifically detects N-Cadherin in WB. It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for N-Cadherin antibody (AA 25-810) (ABIN7872893)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-N-Cadherin Antibody

Binding Specificity: AA 25-810

Purpose: Zebrafish N-cadherin Antibody / Ncad / Cdh2 / Cadherin 2

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish N-cadherin recombinant protein (amino acids M25-R810) was used as the immunogen for the Zebrafish N-cadherin antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish N-cadherin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish N-cadherin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: N-cadherin

Background: N cadherin, also known as Ncad, cadherin 2 and neural cadherin, is a calcium dependent cell adhesion molecule that plays a critical role in the formation and maintenance of tissue architecture during development. In zebrafish, N cadherin is essential for processes such as gastrulation, neurulation, somite formation, and organ morphogenesis.

Zebrafish N cadherin is the ortholog of the human CDH2 gene, which encodes the same protein. Both zebrafish and human N cadherin are members of the classical cadherin family and share a conserved structure that includes extracellular cadherin repeats, a single transmembrane domain, and a cytoplasmic region that interacts with catenins to link cell adhesion to the actin cytoskeleton.

In zebrafish embryos, N cadherin is expressed in the developing nervous system, heart, somites, and other tissues undergoing epithelial to mesenchymal transitions. It mediates homophilic cell-cell adhesion, ensuring coordinated cell movements and stable tissue organization. Loss of N cadherin function in zebrafish results in severe developmental defects, including impaired brain morphogenesis, defective heart formation, and abnormal cell migration.

Due to its strong evolutionary conservation and fundamental role in cell adhesion and signaling, zebrafish N cadherin is widely used as a model to study developmental biology, tissue engineering, cancer metastasis, and congenital disorders involving cadherin dysfunction.

UniProt: Q90275

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane