SCD antibody (AA 26-358)

Catalog No. ABIN7872999

The Rabbit Polyclonal anti-SCD antibody is suitable to detect SCD in samples from Human. It has been validated for WB, ELISA, ICC, IF, IP and FACS.

Quick Overview for SCD antibody (AA 26-358) (ABIN7872999)

Target: SCD (Stearoyl-CoA Desaturase (Delta-9-Desaturase) (SCD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-SCD Antibody

Binding Specificity: AA 26-358

Purpose: SCD1 Antibody / Stearoyl-CoA desaturase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SCD recombinant protein (Position: R26-S358) was used as the immunogen for the SCD1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SCD1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SCD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SCD

Target: SCD (Stearoyl-CoA Desaturase (Delta-9-Desaturase) (SCD))

Alternative Name: SCD1

Background: SCD1 antibody detects Stearoyl-CoA desaturase, an endoplasmic reticulum enzyme that catalyzes the conversion of saturated fatty acids into monounsaturated fatty acids, primarily oleate and palmitoleate. This reaction is essential for maintaining membrane fluidity, lipid signaling, and triglyceride synthesis. The SCD1 antibody is widely used in metabolism and lipid biology research to explore fatty acid desaturation and metabolic regulation.

SCD1 is encoded by the SCD gene located on human chromosome 10q24.31. The enzyme is approximately 41 kilodaltons and contains four transmembrane domains that anchor it to the ER membrane, where it introduces a cis-double bond between the ninth and tenth carbon atoms of saturated fatty acyl-CoAs. The desaturation reaction requires oxygen, NADH, and cytochrome b5, forming the core step in the synthesis of monounsaturated fatty acids.

The SCD1 antibody typically detects a 36-42 kilodalton band by western blot and reveals reticular ER staining in immunofluorescence microscopy. SCD1 plays a vital role in lipid homeostasis by controlling the ratio of saturated to unsaturated fatty acids, influencing membrane composition, lipoprotein secretion, and energy storage.

Upregulation of SCD1 promotes lipogenesis and is associated with obesity, insulin resistance, and hepatic steatosis. Conversely, inhibition or genetic deletion of SCD1 enhances fatty acid oxidation and protects against metabolic disease. SCD1 expression is regulated by sterol regulatory element-binding protein 1c (SREBP1c) and peroxisome proliferator-activated receptors (PPARs), linking it to dietary and hormonal control of lipid metabolism.

Beyond metabolism, SCD1 influences inflammation, cancer cell proliferation, and stem cell maintenance. High SCD1 expression correlates with poor prognosis in several cancers due to its role in sustaining membrane synthesis and redox balance. NSJ Bioreagents provides a validated SCD1 antibody optimized for its applications, supporting research into fatty acid metabolism, energy regulation, and disease mechanisms.

UniProt: O00767

Pathways: Brown Fat Cell Differentiation