FAM59A antibody (AA 277-753)

Catalog No. ABIN7873213

The Rabbit Polyclonal anti-FAM59A antibody (ABIN7873213) specifically detects FAM59A in ELISA, WB, IHC and FACS. The antibody is reactive with Human samples.

Quick Overview for FAM59A antibody (AA 277-753) (ABIN7873213)

Target: FAM59A (Family with Sequence Similarity 59, Member A (FAM59A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAM59A antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-FAM59A Antibody

Binding Specificity: AA 277-753

Purpose: GAREM1 Antibody / GRB2-associated and regulator of MAPK protein 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human GAREM1 recombinant protein (Position: Q277-E753) was used as the immunogen for the GAREM1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GAREM1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GAREM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for FAM59A

Target: FAM59A (Family with Sequence Similarity 59, Member A (FAM59A))

Alternative Name: GAREM1

Background: GAREM1 antibody detects GRB2-associated regulator of MAPK1 subtype 1, an adaptor protein involved in growth factor receptor signaling, particularly within the epidermal growth factor receptor (EGFR) and fibroblast growth factor receptor (FGFR) pathways. GAREM1 acts as a downstream effector of receptor tyrosine kinases and plays a role in mitogen-activated protein kinase (MAPK) signaling, cellular proliferation, and differentiation. The GAREM1 antibody is used to investigate mechanisms of growth factor signaling, cell migration, and oncogenic transformation.

GAREM1 is encoded by the GAREM1 gene located on human chromosome 18p11.32. The protein is approximately 95 kilodaltons and contains multiple functional regions, including a pleckstrin homology (PH) domain, proline-rich motifs, and SH3-binding sequences that facilitate interaction with growth factor receptor-bound protein 2 (GRB2). Through these interactions, GAREM1 acts as a scaffolding adaptor that transmits extracellular growth factor signals to downstream MAPK/ERK cascades, modulating transcriptional responses and cytoskeletal reorganization.

The GAREM1 antibody detects a 90-100 kilodalton protein on western blot and reveals cytoplasmic and perinuclear localization by immunofluorescence. Activation of EGFR or FGFR promotes tyrosine phosphorylation of GAREM1, which enhances its association with signaling molecules such as SHP2, ERK1/2, and SOS1. Functionally, GAREM1 contributes to cell cycle progression and survival, and its overexpression has been observed in certain cancers, including hepatocellular carcinoma and glioblastoma. Depletion or inhibition of GAREM1 disrupts ERK activation, leading to reduced cell proliferation and migration.

GAREM1 also influences differentiation processes by regulating cytoskeletal dynamics and cell adhesion. In neurons, GAREM1 modulates neurite outgrowth and receptor clustering, linking receptor tyrosine kinase signaling to actin remodeling. It may further act as a regulatory hub integrating MAPK and PI3K signaling pathways. NSJ Bioreagents provides a validated GAREM1 antibody optimized for western blot, immunohistochemistry, and immunofluorescence, supporting studies of receptor signaling, cancer cell proliferation, and cellular differentiation.

UniProt: Q9H706

Pathways: EGFR Signaling Pathway