Senataxin (SETX) (AA 28-2447) antibody

Catalog No. ABIN7873246

The Rabbit Polyclonal anti-Senataxin antibody (ABIN7873246) specifically detects Senataxin in WB and ELISA. The antibody is reactive with Mouse and Rat samples.

Quick Overview for Senataxin (SETX) (AA 28-2447) antibody (ABIN7873246)

Target: Senataxin (SETX)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Product Details

Binding Specificity: AA 28-2447

Purpose: SETX Antibody / Senataxin

Purification: Immunogen affinity purified

Immunogen: E.coli-derived mouse SETX recombinant protein (Position: S28-R2447) was used as the immunogen for the SETX antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SETX antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SETX antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details

Target: Senataxin (SETX)

Alternative Name: SETX

Background: SETX antibody detects Senataxin, a nuclear RNA/DNA helicase involved in transcription termination, RNA processing, and DNA damage repair. Encoded by the SETX gene on chromosome 9q34.13, this large helicase contains a conserved C-terminal superfamily 1 (SF1) helicase domain that unwinds RNA/DNA hybrids (R-loops) formed during transcription and replication. By resolving R-loops, SETX maintains genomic stability and coordinates proper transcription termination for RNA polymerase II genes.

Senataxin interacts with transcription termination factors, RNA-processing proteins, and DNA repair complexes to integrate transcriptional and genomic maintenance pathways. It facilitates the repair of transcription-associated DNA damage and supports RNA splicing fidelity by ensuring timely R-loop resolution. SETX localizes to nuclear foci associated with active transcription sites and DNA damage response regions.

The SETX antibody is widely used in molecular genetics, neurobiology, and genome stability research to study helicase activity, R-loop metabolism, and transcriptional control. Western blot analysis identifies a 303 kilodalton band corresponding to Senataxin, while immunofluorescence reveals discrete nuclear foci marking transcriptionally active chromatin. This antibody supports investigations into RNA-DNA hybrid regulation and the prevention of replication stress.

Mutations in SETX cause neurological disorders such as ataxia with oculomotor apraxia type 2 (AOA2) and amyotrophic lateral sclerosis type 4 (ALS4), highlighting its role in maintaining neuronal integrity. Dysfunctional Senataxin results in R-loop accumulation, transcriptional stress, and DNA strand breaks. The SETX antibody provides a reliable reagent for exploring the links between transcription, RNA metabolism, and neurodegeneration. NSJ Bioreagents supplies this antibody validated for its applications, ensuring reproducible detection in genome maintenance studies.

UniProt: A2AKX3