Recombinant Occludin antibody (AA 282-415)

Catalog No. ABIN7873299

The Rabbit Monoclonal anti-Occludin antibody is suitable to detect Occludin in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for Recombinant Occludin antibody (AA 282-415) (ABIN7873299)

Target: Occludin (OCLN)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: OCLN-8526R

Product Details anti-Occludin Antibody

Binding Specificity: AA 282-415

Purpose: Occludin Antibody / OCLN

Purification: Protein A/G affinity

Immunogen: A recombinant human protein fragment (within amino acids 282-415) was used as the immunogen for the Occludin antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the Occludin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Occludin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: Occludin

Background: Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue, in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial Occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C