Senataxin (SETX) (AA 29-2544) antibody

Catalog No. ABIN7873404

The Rabbit Polyclonal anti-Senataxin antibody is suitable to detect Senataxin in samples from Human. It has been validated for WB, ELISA and FACS.

Quick Overview for Senataxin (SETX) (AA 29-2544) antibody (ABIN7873404)

Target: Senataxin (SETX)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details

Binding Specificity: AA 29-2544

Purpose: Senataxin Antibody / SETX

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SETX recombinant protein (Position: E29-R2544) was used as the immunogen for the Senataxin antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Senataxin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Senataxin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details

Target: Senataxin (SETX)

Alternative Name: Senataxin

Background: Senataxin antibody detects DNA/RNA helicase Senataxin, encoded by the SETX gene located on chromosome 9q34.13. Senataxin is a large nuclear helicase that unwinds DNA/RNA hybrids known as R-loops, thereby maintaining genomic stability during transcription and replication. It plays crucial roles in transcription termination, RNA processing, and DNA damage repair, linking transcriptional control with genome maintenance. Senataxin is expressed widely, with elevated levels in neurons, germ cells, and proliferative tissues where transcription-coupled DNA repair is highly active.

Structurally, Senataxin is a 2677-amino-acid helicase belonging to the superfamily 1 (SF1) of DNA/RNA helicases. It contains a C-terminal helicase domain with conserved Walker A and B motifs for ATP binding and hydrolysis, an N-terminal region that mediates protein-protein interactions, and nuclear localization sequences. The helicase activity of Senataxin resolves R-loop structures formed during transcription, preventing collisions between transcription and replication machinery. Co-localization studies show Senataxin enriched in nuclear foci associated with sites of active transcription and DNA repair.

Functionally, Senataxin maintains genome integrity by resolving R-loops and facilitating proper termination of RNA polymerase II transcription. It contributes to DNA double-strand break repair via homologous recombination and regulates transcription-coupled nucleotide excision repair. Senataxin also modulates RNA maturation by interacting with RNA processing factors such as XRN2 and the exosome complex. In neurons, it supports axonal maintenance and stress granule dynamics, while in germ cells, it regulates meiotic recombination and spermatogenesis.

Mutations in the SETX gene are linked to several neurodegenerative and neuromuscular disorders, including ataxia with oculomotor apraxia type 2 (AOA2) and juvenile amyotrophic lateral sclerosis type 4 (ALS4). These conditions are characterized by progressive neurodegeneration and impaired DNA repair capacity. Senataxin deficiency leads to accumulation of R-loops, transcriptional stress, and genomic instability. Pathway associations include RNA processing, DNA damage response, and transcription regulation. During development, Senataxin expression supports neuronal differentiation and genome maintenance in post-mitotic cells.

The Senataxin antibody from NSJ Bioreagents is a valuable reagent for studying transcription-coupled repair, RNA metabolism, and neurodegenerative disease mechanisms.

UniProt: Q7Z333