SCO2 antibody (AA 29-266)

Catalog No. ABIN7873408

The Rabbit Polyclonal anti-SCO2 antibody (ABIN7873408) specifically detects SCO2 in WB, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for SCO2 antibody (AA 29-266) (ABIN7873408)

Target: SCO2 (SCO2 Cytochrome C Oxidase Assembly Protein (SCO2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCO2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SCO2 Antibody

Binding Specificity: AA 29-266

Purpose: SCO2 Antibody / Copper chaperone SCO2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SCO2 recombinant protein (Position: Q29-S266) was used as the immunogen for the SCO2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SCO2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SCO2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SCO2

Target: SCO2 (SCO2 Cytochrome C Oxidase Assembly Protein (SCO2))

Alternative Name: SCO2

Background: SCO2 antibody detects the Copper chaperone SCO2, a mitochondrial inner membrane protein required for the assembly of cytochrome c oxidase (complex IV) of the respiratory chain. The SCO2 gene encodes a metallochaperone that facilitates copper delivery to the COX2 subunit of cytochrome c oxidase, ensuring proper enzyme maturation and electron transport. SCO2 plays an essential role in aerobic energy metabolism, and its deficiency leads to mitochondrial dysfunction and severe metabolic disorders.

Located on chromosome 22q13.33, SCO2 (Synthesis of Cytochrome c Oxidase 2) is one of two homologous human SCO genes, the other being SCO1. Both are nuclear-encoded but localized to mitochondria, where they coordinate copper homeostasis. SCO2 binds copper ions via conserved cysteine and histidine residues and transfers them to cytochrome oxidase subunits through direct protein-protein interactions. Mutations in SCO2 disrupt this process and result in cytochrome c oxidase deficiency, leading to diseases such as fatal infantile cardioencephalomyopathy and Leigh-like syndrome.

SCO2 expression is regulated by hypoxia and cellular copper status. Under low oxygen conditions, transcription factor HIF-1 may influence SCO2 expression to modulate oxidative phosphorylation efficiency. The protein is also involved in maintaining redox balance and preventing the accumulation of reactive oxygen species (ROS) within mitochondria. Beyond its role in energy production, SCO2 contributes to cellular copper distribution and may participate in signaling pathways that coordinate mitochondrial biogenesis.

Immunohistochemical analysis using SCO2 antibody demonstrates mitochondrial localization in heart, skeletal muscle, and brain tissues, consistent with its role in energy metabolism. The antibody serves as a key tool for studying mitochondrial function, copper metabolism, and respiratory chain biogenesis. SCO2 antibody from NSJ Bioreagents provides reliable detection of this essential metallochaperone in biochemical and cell biology research applications.

UniProt: O43819

Pathways: Transition Metal Ion Homeostasis, Warburg Effect