JHDM1D antibody (AA 290-316)

Catalog No. ABIN7873439

This Rabbit Polyclonal antibody specifically detects JHDM1D in WB, FACS and IHC (p). It exhibits reactivity toward Human.

Quick Overview for JHDM1D antibody (AA 290-316) (ABIN7873439)

Target: JHDM1D (Jumonji C Domain-Containing Histone Demethylase 1 Homolog D (JHDM1D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JHDM1D antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-JHDM1D Antibody

Binding Specificity: AA 290-316

Purpose: JHDM1D Antibody / KDM7A

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 290-316 from the human protein was used as the immunogen for the JHDM1D antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the JHDM1D antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the JHDM1D antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for JHDM1D

Target: JHDM1D (Jumonji C Domain-Containing Histone Demethylase 1 Homolog D (JHDM1D))

Alternative Name: JHDM1D

Background: JHDM1D histone demethylase that specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3, thereby playing a central role in histone code. This specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. This demethylates H3K9me2 in absence of H3K4me3.

UniProt: Q6ZMT4