CLCN3 antibody (AA 293-752)

Catalog No. ABIN7873455

The Rabbit Polyclonal anti-CLCN3 antibody is suitable to detect CLCN3 in samples from Rat and Human. It has been validated for WB, IHC, ICC, ELISA, IF and FACS.

Quick Overview for CLCN3 antibody (AA 293-752) (ABIN7873455)

Target: CLCN3 (Chloride Channel 3 (CLCN3))

Reactivity: Rat, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLCN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-CLCN3 Antibody

Binding Specificity: AA 293-752

Purpose: CLCN3 Antibody / Chloride channel protein 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human CLCN3 recombinant protein (Position: G293-R752) was used as the immunogen for the CLCN3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CLCN3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CLCN3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for CLCN3

Target: CLCN3 (Chloride Channel 3 (CLCN3))

Alternative Name: CLCN3

Background: CLCN3 antibody detects Chloride channel protein 3, encoded by the CLCN3 gene on chromosome 4q33. CLCN3 belongs to the CLC family of voltage-gated chloride channels and chloride/proton exchangers, which regulate membrane potential, cell volume, and ion homeostasis. CLCN3 is widely expressed across tissues but is most abundant in the nervous system, kidney, and muscle, where it supports vesicular acidification, endosomal trafficking, and chloride transport across membranes. It plays a vital role in maintaining neuronal excitability, synaptic transmission, and endolysosomal homeostasis.

Structurally, CLCN3 is a dimeric integral membrane protein with each subunit containing its own independent pore. It functions primarily as a Cl?/H+ antiporter rather than a pure chloride channel, coupling chloride efflux to proton influx. This electrogenic exchange contributes to acidification of endosomes and synaptic vesicles, processes essential for neurotransmitter storage and recycling. Mutations or dysregulation of CLCN3 impair endosomal acidification and lead to defective receptor trafficking, altered synaptic vesicle dynamics, and changes in neuronal function.

In the nervous system, CLCN3 contributes to synaptic vesicle function by facilitating neurotransmitter loading and release. Knockout mice lacking CLCN3 display severe neurodegeneration, retinal degeneration, and defects in hippocampal synaptic plasticity. These phenotypes underscore its importance in brain development and function. Additionally, CLCN3 supports cell volume regulation under osmotic stress, protecting neurons and glial cells from swelling-induced injury.

Pathologically, alterations in CLCN3 have been linked to epilepsy, intellectual disability, and neurodegenerative conditions. Some cancer studies have shown that CLCN3 expression is upregulated in gliomas and contributes to tumor growth and invasiveness by regulating intracellular chloride levels and endosomal signaling. As such, CLCN3 has been proposed as a potential therapeutic target in oncology and neurology. Researchers rely on CLCN3 antibody to study its role in these diverse processes, enabling insights into ion channel biology, synaptic function, and disease progression.

Experimentally, CLCN3 antibody has been applied in western blotting to detect the expected ~97 kDa band, in immunofluorescence microscopy to visualize endosomal and vesicular localization, and in immunohistochemistry to track expression in brain and kidney tissue. Co-immunoprecipitation studies using CLCN3 antibody have identified interacting partners including clathrin, synaptophysin, and vesicular proton ATPases, highlighting its integration in vesicular trafficking networks. Functional assays combining CLCN3 antibody with electrophysiological studies allow researchers to correlate protein abundance with transport activity. NSJ Bioreagents provides CLCN3 antibody to support neuroscience, physiology, and cancer research by ensuring reliable detection of this critical ion transporter.

UniProt: P51790