PAX7 antibody (AA 301-505)

Catalog No. ABIN7873712

This Mouse Monoclonal antibody specifically detects PAX7 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for PAX7 antibody (AA 301-505) (ABIN7873712)

Target: PAX7 (Paired Box 7 (PAX7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX7 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM613

Product Details anti-PAX7 Antibody

Binding Specificity: AA 301-505

Purpose: PAX-7 Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant human PAX7 protein corresponding to amino acids 301-505 was used as the immunogen for this PAX-7 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the PAX-7 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the PAX-7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Alternative Name: PAX-7

Background: The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).

UniProt: P23759